STERIS’ VHP M100 Biodecontamination Systems: Closed Loop provide a flexible, safe, environmentally friendly method of decontamination to small, sealed enclosures. STERIS VHP M100 Biodecontamination System utilizes Vaprox? Sterilant (EPA Reg. No. 58779-4) as a means of achieving dry, low temperature sterilization within sealed enclosures such as isolators and glove boxes. The dry, gaseous process consists of four distinct phases: Dehumidification, Conditioning, Biodecontamination, Aeration.